Thermo Fisher, 4440046, TaqMan™ Universal Master Mix II, with UNG

10 x 5 mL TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR). Use TaqMan Universal Master Mix II to amplify complementary DNA (cDNA) and DNA targets for a variety of applications, including quantitation and genotyping.
This TaqMan Universal Master Mix II is supplied as a 2X concentrate and contains:
• AmpliTaq Gold DNA Polymerase, UP (Ultra Pure)
• dNTPs (with dUTP)
• ROX Passive Reference
• Uracil-N-glycosylase (UNG)
• Optimized buffer componentsNote: This TaqMan Universal Master Mix II contains uracil-N-glycoslyase (UNG). Seea complete listing of all TaqMan Universal Master Mix II products, with and without UNG.
TaqMan Universal Master Mix II has been optimized for use with primers and TaqMan probes that have been designed according to Thermo Fisher Scientific guidelines. The master mix can be used with custom TaqMan assays available from our custom assay service, or with pre-optimized assays such as:
• TaqMan gene expression assays
• TaqMan microRNA assays
• TaqMan drug metabolism genotyping assays
• TaqMan SNP genotyping assaysFor RNA quantitation experiments, TaqMan Universal Master Mix II is used in the second step of a two-step reverse transcription/polymerase chain reaction (RT-PCR) protocol. The cDNA template used with the master mix can be generated in a reverse transcription reaction usingcDNA synthesis kitsavailable from Thermo Fisher Scientific.
Alternative product:TryTaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of TaqMan Universal Master Mix II and added additional capabilities for your gene expression analysis.
Concentration: 2X
Fidelity (vs. Taq): 2 X
For Use With (Equipment): 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Passive Reference Dye: ROX (Pre-mixed)
Polymerase: AmpliTaq Gold DNA Polymerase
Product Line: TaqMan™
Product Type: Universal Master Mix II
Purity or Quality Grade: UP (Ultra Pure)
Quantity: 10 x 5 mL
Sample Type: DNA (Genomic), cDNA
Volume: 50 mL
Detection Method: Primer-probe
For Use With (Application): Gene Expression, Genotyping, miRNA analysis
GC-Rich PCR Performance: High
PCR Method: qPCR
Reaction Speed: Standard
Unit Size: 10 x 5 mL