Thermo Fisher, 4464080, Ambion™ mirVana™ miRNA Inhibitor, let-7c positive control

5 nmol miRNA inhibitors are small, chemically modified single-stranded RNA molecules designed to specifically bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity.
We have now released the second generation of miRNA inhibitors,mirVana™ miRNA Inhibitors, which:Display better potency than lead competitor's productOffer maximum and consistent effectin vitroat low miRNA inhibitor concentrationTarget the most updated Sanger contentCan be usedin vitroandin vivo, offering consistency throughout your entire research projectAre non-toxicPositive and negative controls are also available for use along withmirVana™ miRNA Inhibitors in miRNA down-regulation experiments.
mirVana™ miRNA Inhibitor let-7c Positive ControlThemirVana™ miRNA Inhibitor let-7c Positive Control is intended for use as a positive control for mammalian cell culture experiments usingmirVana™ miRNA Inhibitors. Endogenous let-7c miRNA has been shown to negatively regulate HMGA2 mRNA in cultured cells. HMGA2 is a ubiquitously expressed, nonhistone, chromatin protein that can modulate gene expression through changes in chromatin architecture. let-7c miRNA down-regulates levels of HMGA2 mRNA. When transfected into human and mouse cell lines, let-7c miRNA inhibitor blocks endogenous let-7c miRNA, resulting in increased levels of HMGA2 mRNA. Thus, let-7c miRNA inhibitor activity can be monitored in human or mouse cells using real-time reverse transcription PCR (RT-PCR) to detect HMGA2 mRNA.
First Generation Inhibitor ControlsOur first-generation miRNA inhibitors (and controls), Ambion™ Anti-miR™ miRNA Inhibitors, have lower specificity and potency, plus a lower strand specificity compared to the newmirVana™ miRNA Inhibitors. These first-generation inhibitors are recommended for completing ongoingin vitromiRNA studies. For new studies orin vivostudies, we highly recommend our newmirVana™ miRNA Inhibitors and their corresponding controls.
For Use With (Application): miRNA & Non-Coding RNA Analysis
Label or Dye: Unconjugated
Product Line: mirVana™
Product Type: miRNA Inhibitor
Purification Method: Purified
Quantity: 5 nmol
Shipping Condition: Room Temperature
Control Type: Positive Control
RNAi Type: miRNA Inhibitor
Unit Size: Each