Thermo Fisher, 4488593, TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)

200 Reactions TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample. Preamplification enables you to stretch your precious sample into many more real-time PCR reactions.
TaqMan™ PreAmp Master Mix lets you:
• Analyze mRNA from precious samples such as laser capture microdissections, needle biopsies, and formalin-fixed paraffin-embedded (FFPE) tissues
• Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan™ Gene Expression assays
• Analyze up to 100 gene expression targets with minimal hands-on timePreserves Equilibrium of Targets Without Preamplification BiasIn the past, preamplification kits and techniques often resulted in uneven amplification of targets and incorrect or biased data (as shown by low correlation coefficients between preamplified and unamplified samples). TaqMan™ PreAmp Master Mix amplifies with no bias and provides extremely high correlation between amplified and unamplified cDNA.
Easy WorkflowWith TaqMan™ PreAmp Master Mix, preamplification setup takes only a few minutes. After a one-time pooling of the TaqMan™ assays , preamplification setup (mixing the pooled assays with cDNA sample and TaqMan™ PreAmp Master Mix) takes only five additional minutes. Cycling takes 1.
5 hours using a GeneAmp™ 9700 thermal cycler.
For Use With (Equipment): 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Format: Bottle
No. of Reactions: 200 Reactions
Product Line: TaqMan™
Product Type: PreAmp Master Mix
Quantity: 200 reactions
Sample Type: cDNA
Sufficient For: 200 Reactions
Volume: 5 mL
Detection Method: Primer-probe
For Use With (Application): Pre-amplification
GC-Rich PCR Performance: High
PCR Method: qPCR
Reaction Speed: Standard
Unit Size: Each