Thermo Fisher, 44916, Pierce™ Thiophilic Adsorption Kit

Pierce™ Thiophilic Adsorption Kit Thermo Scientific Pierce Thiophilic Adsorbent is a protein-free immunosorbent resin for gentle preferential binding and purification of immunoglobulins from a variety of sources including mouse, rat, rabbit, goat and human. This Pierce Thiophilic Adsorption Kit is a purification kit containing columns pre-packed with the resin and ready-to-use buffers.
Features of Thiophilic Adsorbent:
•High capacity– gently bind and purify approx. 20 mg polyclonal antibody per milliliter of adsorbent resin
•Broad antibody specificity– effective for most immunoglobulin classes and isotypes derived from many different animal species, including chicken IgY
•Simple and fast– quickly fractionate and purify the immunoglobulin component of serum or ascites samples without subsequent dialysis or desalting steps
•Gentle– binding condition is nondenaturing and the elution buffer yields concentrated, salt-free immunoglobulin at near neutral pH; provides excellent recovery of functional antibodyPierce Thiophilic Adsorbent is 6% beaded agarose that has been modified to contain simple sulfone-thioether groups. The result is an affinity resin that has a high binding capacity and a broad specificity for many species of immunoglobulins when incubated in 0.
5M potassium sulfate. In effect, thiophilic adsorption is a resin-based variation of ammonium sulfate precipitation for quick immunoglobulin purification from crude serum samples. Unlike antibody fractionation by ammonium sulfate precipitation, purification with thiophilic adsorbent yields concentrated, essentially salt-free, highly purified immunoglobulins at near-neutral pH. Thus, this simple one-step method eliminates the need for additional treatment of the sample for storage or for subsequent conjugation reactions. The gentle binding and elution conditions ensure a high protein recovery with excellent preservation of antibody activity.
Thiophilic adsorption is based on the ability of some proteins, particularly immunoglobulins, to bind to an immobilized ligand that contains a sulfone group in close proximity to a thioether. Salts that interact with water molecules, such as potassium and ammonium sulfate, promote this binding by driving the protein into close proximity with the ligand.
Description: Pierce Thiophilic Adsorption Kit
Purification Target: IgG Antibody
Quantity: 4 Columns
Column Type: Agarose Resin, Affinity
Format: Gravity Flow Column
Product Line: Pierce™
Product Type: Adsorption Kit
Stationary Phase: Sulfone Thioether
Unit Size: Each