Thermo Fisher, 44999, SulfoLink™ Immobilization Kit for Peptides, 2 mL

SulfoLink™ Immobilization Kit for Peptides, 2 mL Thermo Scientific SulfoLink Immobilization Kit for Peptides, 2 mL is a kit containing all the reagents for preparing the peptide sample for efficient immobilization and five columns containing 2 mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.
Features of SulfoLink Immobilization Kit for Peptides, 2 mL:
•Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
•Fast—spin columns increase protocol speed; prepare and couple peptide samples in 2 hours
•Flexible coupling conditions—use pH 7.
5 to 9.
0 aqueous buffers, organic solvent (e.
g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
•Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
•High capacity—Immobilize 1 to 2 mg of peptide per 2 mL column of SulfoLink Coupling ResinApplications:
• Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
• Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purificationWhen incubated with a solution of peptide that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.
Column Type: Affinity, Agarose Resin
Format: Spin Column
Quantity: 5 Column Kit
Stationary Phase: Haloacetyl
Sufficient For: 1 to 2 mg of Sulfhydryl-Peptide per Column
Capacity (Metric): 2 mL
Product Line: SulfoLink™
Type: Immobilization Kit
Unit Size: Each