Thermo Fisher, 46406, NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer

NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester), mixed isomer Thermo Scientific NHS-Rhodamine is a high-performance derivative of rhodamine dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.
Features of NHS-Rhodamine :
•Amine-specific labeling—NHS-ester varieties of rhodamine efficiently label antibodies and other purified proteins at primary amines (lysine side chains)
•Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescenceNHS Rhodamine is an amine-reactive derivative of rhodamine dye that has wide-ranging application as antibody and other probe labels for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as western blotting and ELISA.
Applications:
• Label antibodies for use as immunofluorescent probes
• Label oligonucleotides for hybridization probes
• Detect proteins in gels and on western blotsProperties of Rhodamine Dyes:Thermo Scientific Pierce Rhodamine Dyes are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra, and for protein applications there is no need to isolate a specific isomer.
NHS-Rhodamine is activated with the N-hydroxy-succinimidyl-ester (NHS ester) functional group. Compared to TRITC, the NHS-ester deriviative has greater specificity toward primary amines in the presence of other nucleophiles and results in a more stable linkage following labeling.
Application Data:Chemical Reactivity: Amine
Description: Pierce NHS-Rhodamine
Emission: 575
Excitation: 552
Label or Dye: Rhodamine Isomers, TMR (Tetramethylrhodamine), TRITC Isomers
Product Type: NHS-Rhodamine
Quantity: 25 mg
Reactive Moiety: Active Ester, Succinimidyl Ester, NHS Ester
Solubility: DMF (Dimethylformamide)
Label Type: Classic Dyes
Product Line: Pierce™
Unit Size: Each