Thermo Fisher, 46618, DyLight™ 680 Maleimide

DyLight™ 680 Maleimide Thermo Scientific DyLight 680 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 680 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes.
DyLight 680produces near-infrared (IR) fluorescence that replaces other near-IR dyes, including Cy5.
5™ dye and Alexa Fluor™ 680, and is ideal for multi-color applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.
Features of DyLight 680 Maleimide:
•High performance—DyLight 680 fluoresces brighter than Alexa Fluor 680 and Cy5.
5 dyes
•Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
•Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
•Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiencyApplications:
• Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategiesDyLight 680 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.
5-7.
5. Learn more aboutmaleimide chemistry.
Chemical Reactivity: Thiol
Description: DyLight 680 Maleimide
Emission: 712
Excitation: 692
Label or Dye: DyLight™ 680
Product Type: Maleimide
Quantity: 1 mg
Reactive Moiety: Maleimide
Solubility: DMF (Dimethylformamide)
Label Type: DyLight Fluors
Product Line: DyLight™
Unit Size: Each