Thermo Fisher, 46621, DyLight™ 800 Maleimide

DyLight™ 800 Maleimide Thermo Scientific DyLight 800 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 800 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes.
DyLight 800is a near-IR fluor that is invisible to the naked eye but increases the staining options when using infrared imaging systems. DyLight 800 has spectral properties that are very similar to other near-IR dyes, including Alexa Fluor™ 790 and IRDye™ 800. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.
Features of DyLight 800 Maleimide:
•High performance—DyLight 800 replaces Alexa Fluor 800 and IRDye 800 for near-infrared staining
•Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
•Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
•Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiencyApplications:
• Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting, and ELISA assay
• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategiesDyLight 800 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.
5-7.
5. Learn more aboutmaleimide chemistry.
Chemical Reactivity: Thiol
Description: DyLight 800 Maleimide
Emission: 794
Excitation: 777
Label or Dye: DyLight™ 800
Product Type: Dye
Quantity: 1 mg
Reactive Moiety: Maleimide
Solubility: DMF (Dimethylformamide)
Label Type: DyLight Fluors
Product Line: DyLight™
Unit Size: Each