Thermo Fisher, 53116, Pierce™ Streptavidin Plus UltraLink™ Resin Thermo Scientific™

Pierce™ Streptavidin Plus UltraLink™ Resin Thermo Scientific™ The Thermo Scientific™ Pierce™ High Capacity Streptavidin Chromatography Cartridges are convenient, ready-to-use devices for separating biotinylated molecules from non-biotinylated molecules and for purifying antigens using biotinylated antibodies. The chromatography cartridges contain a beaded resin of immobilized recombinant streptavidin protein that binds biotinylated proteins at greater than 10 mg/mL of beads. This corresponds to 2 to 3 times higher biotin-binding capacity than products from other suppliers and is ideal when a high amount of recovery is required.
These cartridges enable fast, easy, and reproducible chromatographic separations and are compatible with the major automated liquid-chromatography systems or manual syringe processing (see Table 1 for cartridge general properties). The cartridges attach directly to ÄKTA™ or FPLC systems without additional connectors. The included accessory pack readily adapts cartridges for use with Luer-Lok™ syringe fittings or 1/16' tubing. These cartridges can be regenerated multiple times for affinity purification.
Features of High Capacity Streptavidin Agarose:
•Streptavidin—purified recombinant streptavidin protein (53 kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule
•Agarose resin—support is cross-linked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
•Inert and stable—streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
•High capacity—this variety of beads has a dense load of immobilized streptavidin, providing a binding capacity greater than 10 mg of biotinylated BSA per mL of resin
•Two formats—available in several bottle sizes of resin slurry and as packed 1 mL and 5 mL chromatography cartridgesProperties of cross-linked 6% beaded agarose (CL-6B):
• Support pH stability: 2 to 14 (short term); 3 to 13 (long term)
• Average particle size: 45 to 165 microns
• Exclusion limit: 10,000 to 4,000,000 daltons
• Maximum recommended flow rate: 4 mL/minute (1 mL column), 5 mL/min (5 mL column)
• Maximum linear velocity: 30 cm per hour
• Maximum pressure: 0.
3 MPa, 43.
5 psi or 3 barPierce High Capacity Streptavidin Chromatography Cartridges consist of purified recombinant streptavidin covalently immobilized at a high density onto high-quality cross-linked 6% beaded agarose. Biotinylated antibodies, proteins, peptides, nucleic acids, and other molecules or interaction complexes can be captured, immunoprecipitated, removed, or purified from samples using this streptavidin resin.
Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein fromStreptomyces avidiniiis carbohydrate-free, has an acidic pI of 5.
5 and a mass of 75 kDa. The recombinant protein used to manufacture Pierce streptavidin resins has a near-neutral isoelectric point and a mass of 53 kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates.
For more information see:Chromatography Cartridges for Desalting and Affinity PurificationColumn Type: Affinity, UltraLink™ Resin
Format: Bottle
Protein Form: Recombinant
Quantity: 2 mL
Stationary Phase: Streptavidin
Sufficient For: Binding ≥/= 48 μg Biotin/mL Resin (≥/= 4 mg Biotinylated BSA/mL Resin)
Target: Biotin-Tagged Molecule
Capacity (Metric): 10 mg Biotinylated BSA Binding per mL Resin
Particle Size: 45 to 165 μm
Product Line: Pierce™, UltraLink™
Type: Resin
Unit Size: Each