Thermo Fisher, 61870143, RPMI 1640 Medium, GlutaMAX™ Supplement

5 L RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This RPMI 1640 is modified as follows:WithWithout
• GlutaMAX™
• HEPES
• Phenol RedThe completeformulationis available.
Using RPMI 1640 MediumRPMI 1640 medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 medium withGlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. RPMI 1640 medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 medium requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (2.
0 g/L) and therefore requires a 5–10% CO2environment to maintain physiological pH.
cGMP manufacturing and quality systemRPMI 1640 is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (61870-010). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.
Cell Line: HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell Type: Leukemic Cells
Concentration: 1 X
Product Line: Gibco™, GlutaMAX™, SynerGy™
Product Type: RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity: 5 L
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Liquid
Serum Level: Standard Serum Supplementation
Sterility: Sterile-filtered
With Additives: GlutaMAX, Phenol Red
Without Additives: No HEPES, No Sodium Pyruvate
Unit Size: Each