Thermo Fisher, 62274, DyLight™ 650-4xPEG NHS Ester

DyLight™ 650-4xPEG NHS Ester Thermo Scientific DyLight 650-4xPEG Amine-Reactive Dye is a derivative of our high-performanceDyLight 650 Dyethat can be used to fluorescently label antibodies and other proteins for far-red detection.
The DyLight 650-4xPEG dye contains 4 polyethylene glycol (PEG) chains that are non-cytotoxic, enhance fluorescence, and reduce nonspecific binding of conjugates made with them. Conjugates made with DyLight 650-4xPEG Dye can be used as molecular probes for cellular imaging and other fluorescence detection methods. The far-red to NIR fluorescence properties of DyLight 650-4xPEG Dye make it especially useful in a variety of biological, chemical, and pharmaceutical applications, includingin vivoimaging. The PEG chains also improve solubility of the dyes and labeled molecules in aqueous solution, aid in cell permeability, and improve tissue retention.
Features of DyLight 650-4xPEG NHS Ester:
•High fluorescence intensity—significantly brighter fluorescence than Alexa Fluor™ 647
•PEGylated—improves solubility in aqueous solution and aids in cell permeabilityApplications:
• Fluorescence microscopy
•In vivoorex vivoimaging
• Cell-based assays
• Flow cytometry/fluorescence-activated cell sorting (FACS)DyLight 650-4xPEG Amine-Reactive Dye is activated with anN-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more aboutNHS ester chemistry.
Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to the labeling reagent.
Chemical Reactivity: Amine
Emission: 672
Excitation: 652
Label or Dye: DyLight™ 650
Product Type: Dye
Quantity: 1 mg
Reactive Moiety: Active Ester, Succinimidyl Ester, NHS Ester
Solubility: DMF (Dimethylformamide)
Label Type: DyLight Fluors
Product Line: DyLight™
Unit Size: Each