Thermo Fisher, 65-0859-70, eBioscience™ Calcium Sensor Dye eFluor™ 514

Thermo Fisher, 65-0859-70, eBioscience™ Calcium Sensor Dye eFluor™ 514 eFluor™ 514 Calcium Sensor Dye is a membrane-permeable dye used for monitoring changes in intracellular free calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Once eFluor™ 514 Calcium Sensor Dye enters the cell, cellular esterases cleave the AM group yielding a membrane-impermeable dye fluorescing at ∼520 nm . eFluor™ 514 Calcium Sensor Dye, like Fluo-3 and Fluo-4, is a commonly used dye among the visible light-excitable calcium indicators but with increased cellular uptake (even at room temperature) and brightness.
calcium binding affinity: Kd = 232 nMMolecular Weight: approximately 1100 DaltonsPeak Excitation: 490 nmPeak Emission: 514 nmeFluor™ 514 Calcium Sensor Dye should be reconstituted in high-quality, freshly opened DMSO. Recommended concentration: 2-5 mM.
Once reconstituted, it should be protected from light and stored dessicated at -20°C; avoid freeze-thawing.
Reported ApplicationFlow Cytometric Analysis, MicroscopyDetection Method: Fluorescence
Quantity: 10 x 50μg
Emission: 490/514
For Use With (Application): Calcium Assay
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Product Line: eBioscience
Product Type: Dye
Unit Size: Each