Thermo Fisher, 78205.10.ML, ExoSAP-IT™ PCR Product Cleanup Reagent

ExoSAP-IT™ PCR Product Clean-up Reagent, 5000 rxns, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification, Store at –5 to °30°C, PCR Purification, 5000 rxns, 10 mL ExoSAP-IT™ PCR Product Cleanup Reagent is used for enzymatic cleanup of amplified PCR product. It hydrolyzes excess primers and nucleotides in a single step. ExoSAP-IT-purified samples are ready for use in downstream applications such as DNA sequencing or single nucleotide polymorphism (SNP) analysis.
Remove excess primers and unincorporated nucleotides enzymaticallyPrepare DNA sequencing samples with one pipetting stepConserve precious PCR products with up to 100% recoveryMinimize column cleanup and sample lossThe ExoSAP-IT one-step solution provides a simple way to enzymatically purify a PCR reaction. The reaction setup is complete with one pipetting step, which is followed by two incubations. The first incubation digests excess primer and dephosphorylates nucleotides. The second, high-temperature incubation inactivates the enzymes.
Enzymatic cleanup is a very efficient method for PCR purification. Adding ExoSAP-IT reagent directly to the PCR product eliminates transfer steps to tubes, wells, or columns and conserves PCR amplicons, helping reduce the chance of cross contamination; no further processing needed.
After enzymatic cleanup with ExoSAP-IT reagent, primers and dNTPs will no longer interfere with DNA sequencing or SNP analysis.
Description: ExoSAP-IT™ PCR Product Clean-up Reagent, 5000 Reactions, Recombinant, Dry Ice, DNA Sequencing or Single Nucleotide Polymorphism (SNP) Analysis, Use with PCR Purification
For Use With (Application): PCR Purification
No. of Reactions: 5000 Reactions
Product Line: ExoSAP-IT™
Protein Form: Recombinant
Quantity: 10 mL
Research Category: Genetic Diseases
Sample Type: PCR Products
Shipping Condition: Dry Ice
Unit Size: Each