Thermo Fisher, 785951000RXN, HT ExoSAP-IT™ Fast High-Throughput PCR Product Cleanup

Fast High-Throughput PCR Product Clean-up, 1000 rxns, Highly Stable, 4 Minute Protocol, Ideal for High-throughput PCR Product Clean-up Using Liquid Handling Platforms and Multi-Channel Pipettes, For high-throughput PCR product clean-up using liquid handling platforms and multi-channel pipettes, 1000 HT ExoSAP-ITFastHigh-Throughput PCR Product Cleanup, with its shortened 14 minute protocol, is a highly stable, customized formulation of the original ExoSAP-IT reagent. It is ideal for high-throughput PCR product cleanup using liquid handling platforms and multi-channel pipettes. Multiple packaging formats (vials, 8-tube strips, 96-well plates) ensure ease of use and flexibility in experimental design.
Like the original formulation, HT ExoSAP-ITFastreagent is a mixture of Exonuclease I and Shrimp Alkaline Phosphatase for the enzymatic removal of excess primers and dNTPs following a PCR reaction. This enzymatic cleanup reagent has a decreased viscosity yet maintains the same convenience and stability that you have come to expect from the ExoSAP-IT product line. As with all products in the ExoSAP-IT family, HT ExoSAP-ITFastreagent allows PCR cleanup with a single pipetting step. .
HT ExoSAP-ITFastreagent is scalable for PCR cleanup from a single tube up to a 384-well plate PCR reaction.
•14 minute protocol is half the time of standard protocols
•One-tube PCR cleanup — add HT ExoSAP-ITFastreagent directly to PCR product
•100% sample recovery — no loss of PCR products regardless of the fragment size
•Stable at 4°C for one month
•Removes excess primers and dNTPs — does not interfere with downstream applications-sequencing-SNP analysis-single base extension-fragment analysisAdding to our family of patented ExoSAP-IT products, HT ExoSAP-ITFastHigh-Throughput PCR Cleanup reagent quickly provides accurate and consistent results in high-throughput applications. HT ExoSAP-ITFastreagent is over 50% faster than standard HT ExoSAP-IT reagent with a 14 minute total protocol time.
•Add it directly to the PCR product and incubate at 37°C for just 7 minutes.
• After PCR treatment, inactivate it by simply heating to 80°C for another 7 minutes.
This product is ideal for high volume labs that require quick sample turn around. With such a brief and simple protocol, proper sample cleanup never needs to be sacrificed due to processing or instrument time constraints. Ensure the highest quality Sanger sequencing results while keeping sample processing moving quickly and efficiently.
PCR products cleaned up with HT ExoSAP-ITFastreagent prior to automated sequencing displayed superior sequencing results compared to the untreated equivalent (Fig. 2).
HT ExoSAP-ITFastreagent displayed excellent stability in all formats tested including vials, 8-tube strips, and 96-well plates. The product showed no loss in function after 10 freeze thaw cycles (Fig. 3), and is stable at variable temperatures for extended periods of time compared to enzyme mix equivalents. With excellent stability and a greater than 50% faster protocol, HT ExoSAP-ITFastreagent is ideal for high-throughput assays where robotic platforms are utilized and large volumes of samples are processed in successive testing runs.
Collectively, these results clearly demonstrate the effectiveness of HT ExoSAP-ITFastreagent to ensure the highest quality sequences while keeping sample processing moving quickly and efficiently.
Format: 8 Tube Strip, 96-well Plate, Vial
Final Product Type: Fast High-Throughput PCR Product Clean-up
For Use With (Application): For high-throughput PCR product clean-up using liquid handling platforms and multi-channel pipettes
No. of Reactions: 1000 reactions
Product Type: HT ExoSAP-IT™ Fast High-Throughput PCR Product Clean-up
Quantity: Each
Test Time: 14 min
Unit Size: Each