Thermo Fisher, 88370, DMEM:F-12 for SILAC

DMEM:F-12 for SILAC Thermo Scientific™ DMEM:F-12 for SILAC is optimized for use with stable isotope labeling with amino acids in cell culture (SILAC) to analyze protein expression by mass spectrometry (MS).
Features of DMEM:F-12 for SILAC:
• Flexible—liquid media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through multiple isotopic amino acid labeling
• High quality—medium is sterile, endotoxin-free, and cell culture-compatibleSILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization, and quantitation of proteins.
This SILAC medium is used together with heavy and light amino acids for the differential isotopic labeling of cells for SILAC analysis. SILAC media supplemented with heavy amino acids are capable of nearly 100% label incorporation into proteins of living cells.
Related productsNeuCode™ Lysine -080, 50 mgFetal Bovine Serum, dialyzed, US originDMEM for SILACFinal Product Type: Liquid Medium
For Use With (Equipment): Mass Spectrometer
Label or Dye: Mass Isotope of Amino Acid
Quantity: 500 mL
Shipping Condition: Room Temperature
Workflow Step: Cell Culture
Detection Method: Mass Spectrometry
Product Type: Media
Starting Material: Protein Samples
Unit Size: Each