Thermo Fisher, 8842-6599-45, eBioscience™ BrdU Kit for IHC/ICC Immunofluorescence eFluor™ 615

eBioscience™ BrdU Kit for IHC/ICC Immunofluorescence eFluor™ 615 This BrdU Kit for immunohistochemistry (IHC)/immunocytochemistry (ICC) with immunofluorescence detection contains the necessary reagents and buffers for identifying and examining proliferating cells by immunohistochemical or immunocytochemical analysis. Cells are labeled in vitro or in vivo with 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine, which is incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Following fixation and antigen retrieval steps, cells or tissue sections are stained for BrdU incorporation and visualized using a streptavidin-conjugated fluorophore. This kit has been optimized for IHC with both frozen and formalin-fixed paraffin embedded BrdU-labeled mouse intestine and ICC of BrdU-pulsed HeLa cells grown on culture slides.
ConjugateeFluor 615Emit615 nmExcite595 nmReported ApplicationImmunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, Immunohistochemical Staining of Frozen Tissue Sections, Microscopy, ImmunocytochemistryDetection Method: Fluorometric
For Use With (Application): ICC (Immunochemistry), IHC (Immunohistochemistry)
Product Type: IHC/ICC BrdU Kit
Quantity: 50 tests
Target Species: All species
Conjugate: BrdU
Product Line: eBioscience™
Unit Size: Each