Thermo Fisher, 88838, Pierce™ HA-Tag Magnetic IP/Co-IP Kit

Pierce™ HA-Tag Magnetic IP/Co-IP Kit The Thermo Scientific Pierce Magnetic HA-Tag IP/Co-IP Kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of HA fusion proteins or co-IP experiments using HA-tagged bait proteins.
Features of the HA-Tag Magnetic IP/Co-IP Kit:Specific magnetic beads—covalently immobilized high-quality anti-HA monoclonal antibody enables high yields of immunoprecipitation productsLow non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target bindingTrouble-free elution—low-pH elution buffer ensures recovery of HA-tagged protein interaction complexes without antibody leaching contaminationConvenient and fast—complete kit and easy-to-follow instructions provide optimized protocols to perform IP or Co-IP experiment in approximately 1 hourVersatile—magnetic beads are compatible with manual and automated magnetic separation workflows (e.
g., Thermo Scientific KingFisher Instruments)Unlike traditional IP procedures based on capture with Protein A/G beads, this kit uses magnetic beads containing pre-immobilized anti-HA antibody. These Pierce Anti-HA Magnetic Beads ensure specific binding of HA-tagged protein complexes from biological samples. Because the antibody is covalently attached to the beads, IP-targets are easily eluted and recovered without antibody contamination. The complete IP kit includes the magnetic beads, lysis/wash buffer, low-pH elution buffer, neutralization buffer, HA-tag positive control lysate, and non-reducing sample buffer for SDS-PAGE. Protocols are provided for both manual and automated magnetic separation workflows. Sufficient components are provided to perform 40 IP or co-IP assays.
The hemagglutinin (HA) peptide (YPYDVPDYA), derived from the human influenza virus HA protein, is one of several fusion protein tags used for recombinant protein expression. The Pierce Magnetic HA-Tag IP/Co-IP Kit uses a specific, high-affinity immobilized antibody (clone 2-2.
2.
14) for rapid purification of HA-tagged fusion proteins from bacterial and mammalian cell lysates, as well as from lysates prepared with the Pierce Humanin vitroTranslation Kits. The beads are incubated with a cell lysate containing HA-tagged protein, the fusion protein is captured, and the beads are subsequently washed and then eluted using low-pH elution buffer or non-reducing sample buffer. The protocol and buffers have been optimized for both IP and co-IP reactions, enriching for specific protein interaction complexes in the eluted samples. Anti-HA antibody can be used to detect HA-tagged protein by Western blot analysis.
For information about the binding capacity and other properties of the magnetic beads used in this kit, seePierce Anti-HA Magnetic Beads (Part No. 88836).
Assay: Affinity Purification
For Use With (Application): Pull-Down (Tag-Based), Immunoprecipitation (IP)
For Use With (Equipment): KingFisher™ Flex, Magnetic Stand
Format: Kit
Formulation: Slurry: 10 mg/mL, 1% solids
Quantity: 40 reactions
Sufficient For: 40 Reactions
Target: Target-Specific
Capacity (Metric): 1 mL
Product Line: Pierce™
Type: Tag IP/Co-IP Kit
Unit Size: Each