Thermo Fisher, 88900, NHS-Phosphine

NHS-Phosphine Thermo Scientific NHS-Phosphine is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.
Features of NHS-Phosphine:
•Soluble—reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
•Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
•Specific—NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
•Chemoselective—azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiencyThis NHS-ester compound reacts to form covalent bonds with primary amines (e.
g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine group (PPh2) reacts with azide-labeled molecules by a mechanism known asStaudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, this NHS-Phosphine reagent facilitates a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.
Cell Permeability: Not determined
Description: NHS-Phosphine
Form: Powder
Labeling Method: Chemical Labeling
Molecular Weight (g/mol): 461.
4
PEGylated: No
Product Line: Pierce™
Quantity: 10 mg
Reactive Moiety: NHS Ester, Phosphine
Shipping Condition: Ambient
Solubility: DMF, DMSO
Spacer Arm Length: 5.
4 Å
Water Soluble: No
Chemical Reactivity: Amine-Azide
Cleavable: No
Crosslinker Type: Heterobifunctional
Format: Standard
Product Type: Crosslinker
Spacer: Short (<10 Å)
Unit Size: Each