Thermo Fisher, 89904, Sodium Deoxycholate Detergent Thermo Scientific™

Sodium Deoxycholate Detergent Thermo Scientific™ Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions.
Features of Sodium Deoxycholate:
• Popular anionic, bile-acid detergent for many laboratory uses
• Effective in disrupting and dissociating many types of protein interaction
• Can be removed from solution by dialysis
• Useful for elution or regeneration of certain kinds of affinity columns
• High-purity compound with low UV absorptivityProperties of Sodium Deoxycholate:
• Alternative Names: Sodium deoxycholic acid; deoxycholate, sodium salt
• Chemical Name: 3, 12-α-Dihydroxy-5β-cholan-24-oic acid, monosodium salt
• Molecular Weight: 414.
6g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 5 (average)
• Micelle Molecular Weight: 2000g (average)
• Critical Micelle Concentration (CMC): 2 to 6 mM (0.
083 to 0.
249%, w/v)
• Cloud Point: Unknown
• Dialyzable: YesSpecifications for Sodium Deoxycholate (Part No. 89904, 89905):
• Formula: C24H39O4Na
• Molecular Weight: 414.
6g
• Purity (by HPLC): ≥98%
• Absorbance (1% Detergent Solution): 340nm <0.
02; 280nm <0.
04; 260nm <0.
06
• pH (1% Solution): 5 to 9
• Solubility (in water at 20°C): ≥5%Sodium deoxycholate (deoxycholic acid) is a water-soluble, bile-acid, ionic detergent commonly used in protein methods. It is most frequently used as a component of cell lysis buffers (e.
g., RIPA buffer), but also has been used for liposome preparation, isolation of membrane proteins and lipids, preventing non-specific binding in affinity chromatography and a cell culture media supplement.
The effectiveness of a detergent in any application depend upon the detergent's concentration. Too much or too little detergent can often have a deleterious effect. It is recommended that you examine a variety of detergent concentrations in your application. At concentrations above 2 mM, cholate will form micelles having MW ∼2000. The small micelle size allows easy removal by dialysis or gel filtration when needed. Note: removal of a detergent from a protein solution may result in protein precipitation and/or aggregation.
Sodium deoxycholate is the detergent recommended for stripping endotoxin (Lipopolysaccharide or LPS) from immobilized Polymyxin B columns. This is the recommended product for use with the Thermo Scientific Detoxi-Gel Endotoxin Removing Gel.
Quantity: 5 g
Reagent Type: Detergent (Pure)
Form: Powder
Product Type: Detergent
Unit Size: Each