Thermo Fisher, A1049001, MEM α, nucleosides, no ascorbic acid

MEM α, nucleosides, no ascorbic acid MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications. Find the right formulation using themedia selector tool.
This MEM α is modified as follows:WithWithoutDeoxyribonucleosidesAscorbic acidRibonucleosidesL-glutaminePhenol RedThe completeformulationis available.
Using MEM αMEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This MEM is modified without ascorbic acid. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10%Fetal Bovine Serum(FBS). MEM α uses a sodium bicarbonate buffer system (2.
2 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH.
Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Cell Type: Primary Rat Astrocytes
Concentration: 1 X
Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
Product Line: Gibco™
Product Type: MEM α (Minimum Essential Medium α)
Quantity: 500 mL
Shelf Life: 12 Months From Date of Manufacture
Shipping Condition: Room Temperature
Classification: Animal Origin-free
Form: Liquid
Serum Level: Standard Serum Supplementation
Sterility: Sterile-filtered
With Additives: Low Glucose, Glutamine, Phenol Red, Sodium Pyruvate, Deoxyribonucleosides, Ribonucleosides
Without Additives: No Ascorbic Acid
Unit Size: Each