Product Description
Puromycin Dihydrochloride Puromycin Dihydrochloride is an aminonucleoside antibiotic produced byStreptomyces alboniger. Puromycin works by inhibiting peptidyl transfer on both prokaryotic and eukaryotic ribosomes; resistance is conferred by the expression of thepacgene.
Puromycin is widely used in cell biology as a selection antibiotic agent in mammalian cell culture systems. The recommended working concentration ranges from 0.
2–5.
0 μg/mL, although it can be toxic to eukaryotic cells at concentrations as low as 1 μg/mL. Gibco™ Puromycin Dihydrochloride is supplied at 10 mg/mL in 20 mM HEPES buffer (pH 6.
2–6.
8) in 10 vials each containing 1 mL.
Other Choices and More InformationWe offer a wide range of antibiotics and antimycotics in both powder and liquid formats.
See the complete list, or find products for:
•Contamination control
•Eukaryotic and bacterial selectionSee recommendations forworking concentrations for selection antibiotics.
Learn more about theuse of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.
Concentration: 10 mg/mL
Culture Type: Mammalian Cell Culture, Insect Cell Culture
For Use With (Application): Eukaryotic Selection⁄Stable Cell Line Generation
Product Line: Gibco™
Quantity: 10 x 1 mL
Shelf Life: 12 Months
Shipping Condition: Dry Ice
Form: Liquid
Product Type: Antibiotic
Sterility: Sterile-filtered
With Additives: HEPES
Unit Size: Each
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Collaboration
Tony Tang
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