BRAND / VENDOR: Thermo Fisher

Thermo Fisher, A11144, ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit

CATALOG NUMBER: A11144

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Product Description

ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit The ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ tetracycline-regulated lentiviral expression vector, pLenti6.
3/TO/V5-DEST™ for easy recombination-based cloning and lentiviral-based, regulated, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.
3/TO/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
AdvantagesGenerates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cellsEasy recombination-based cloning using Gateway™ technologyStable, long-term, tetracycline-regulated expressionEnhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systemsKey FeaturesWPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target geneBlasticidin selection marker for stable selection under control of SV40 promoterKit includespLenti6.
3/TO/V5-DEST™ vectorpLenti3.
3/TR vectorpLenti6.
3/TO/V5-GW/lacZ plasmidTetracyclineOne Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)pENTRTM Gus positive control plasmid.
Antibiotic Resistance Bacterial: Ampicillin (AmpR), Blasticidin (BsdR)
Cleavage: No Cleavage Site
Constitutive or Inducible System: Inducible
Delivery Type: Lentiviral
For Use With (Application): Viral Expression
Gateway Enzyme: LR Clonase™ II Plus, LR Clonase™ II
Inducing Agent: Doxycycline, Tetracycline
PCR Enzyme: Proof-Reading Polymerase
Product Type: Vector Kit
Protein Tag Position (to your gene): C-terminal
Quantity: 20 Reactions
Replication Origin: pUC Origin
Selection Agent (Eukaryotic): Blasticidin
Selection Marker Eukaryotic: BsdR
Selection Marker Promoter: PGK Promoter
Special Elements: WPRE, cPPT
Vector: pLenti
Cloning Method: Gateway
Product Line: Gateway™, HiPerform™, T-REx™, ViraPower™
Promoter: CMV/TO
Protein Tag: V5 Epitope Tag
Unit Size: Each

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