BRAND / VENDOR: Thermo Fisher

Thermo Fisher, A11146, ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit

CATALOG NUMBER: A11146

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Product Description

ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit The ViraPower™ HiPerform™ Promoterless Gateway™ Vector Kit contains the MultiSite Gateway™-adapted ViraPower™ HiPerform™ promoterless lentiviral expression vector, pLenti6.
4/R4R2/V5-DEST™ for easy recombination-based cloning and lentiviral-based high-level expression of a target gene from any promoter of choice in dividing and non-dividing mammalian cells. The pLenti6.
4/R4R2/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
AdvantagesGenerates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cellsEasy, simultaneous, recombination-based cloning of multiple DNA fragments in a defined order and orientation using MultiSite Gateway™ technologyExpression of the target gene under the control of a promoter of choiceStable, long-term expressionEnhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systemsKey FeaturesWPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
Promoterless vector to express the target gene under the control of a promoter of choiceBlasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expressionKit includespLenti6.
4/R4R2/V5-DEST™ KitpENTR™ 5' TOPO™ TA Cloning Kit (Cat. No. K59120)One Shot™ Stbl3™ Chemically CompetentE. coli(Cat. No. C737303)pENTR™ Gus positive control plasmidAntibiotic Resistance Bacterial: Ampicillin (AmpR), Blasticidin (BsdR)
Cleavage: No Cleavage Site
Constitutive or Inducible System: Constitutive
Delivery Type: Lentiviral
For Use With (Application): Clone your own Promoter, Viral Expression
Gateway Enzyme: LR Clonase™ II Plus, LR Clonase™ II
PCR Enzyme: Proof-Reading Polymerase
Product Type: Vector Kit
Protein Tag Position (to your gene): C-terminal
Quantity: 20 Reactions
Replication Origin: pUC Origin
Selection Agent (Eukaryotic): Blasticidin
Selection Marker Eukaryotic: BsdR
Selection Marker Promoter: PGK Promoter
Special Elements: WPRE, cPPT
Vector: pLenti
Cloning Method: Gateway
Product Line: Gateway™, HiPerform™, ViraPower™
Promoter: None (Promoterless), EF-1α, CMV
Protein Tag: V5 Epitope Tag
Unit Size: Each

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