Thermo Fisher, A15005, pJTI™ R4 EXP CMV-TO EmGFP pA Vector

pJTI™ R4 EXP CMV-TO EmGFP pA Vector The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection, the EmGFP can be inducibly expressed with doxycycline and the successfully retargeted cells will fluoresce green.
Ensure the Success of Your Jump-In™ T-REx™ Retargeting ReactionsSuccessful retargeting of Jump-In™ T-REx™ parental cell lines like theJump-In™ T-REx™ HEK293 Kitis dependent on a variety of factors, such as:
• Transfection efficiency
• Cell confluency
• Antibiotic selection conditions
• Quality and concentration of DNA
• Retargeting vector to integrase vector ratioWe strongly recommend including a positive control retargeting reaction using the pJTI™ R4 Exp CMV-TO EmGFP pA vector in your Jump-In™ experiment, along with negative controls (no plasmid DNA, no integrase vector), so you can easily visualize the results and optimize the retargeting conditions.
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Constitutive or Inducible System: Inducible
Delivery Type: Transfection
For Use With (Application): Stable Cell Line Development, Targeted Integration, Regulated Expression
Inducing Agent: Tetracycline
Product Type: Mammalian Expression Vector
Quantity: 100 μg
Reporter Gene: GFP (EmGFP)
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Vector: Jump-In Vectors
Product Line: Jump-In™
Promoter: CMV/TO
Protein Tag: GFP (EmGFP)
Unit Size: Each