Product Description
LanthaScreen™ TR-FRET LXR beta Coactivator Assay Kit, rabbit This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4658:The LanthaScreen™ TR-FRET LXR (liver X receptor) beta Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR beta ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR beta ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.
How it works:In theLanthaScreen™ TR-FRET LXR beta Coactivator Assay, LXR beta-LBD is added to ligand test compounds followed by the addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the LXR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
Contents and Storage:TheLanthaScreen™ TR-FRET LXR beta Coactivator Assay Kitcontains LXR beta-LBD (GST) protein, fluorescently labeled D22 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Assay Category: Biochemical Assay
Color: Green
Detection Method: Fluorescence
Druggable Target: Nuclear Receptors
For Use With (Application): Nuclear Receptor Assay, TR-FRET
For Use With (Equipment): Microplate Reader
Ligand: LXR Beta
No. of Assays: 800 x 20 μL assays
Packaging: 384-well Plate
Product Type: TR-FRET LXR Beta Coactivator Assay Kit
Quantity: 800 x 20 μL assays
Readout: TR-FRET (Activity)
Conjugate: Tb (Terbium)
Product Line: LanthaScreen™
Unit Size: Each
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Collaboration
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