Thermo Fisher, A21676, ARES™ Alexa Fluor™ 647 DNA Labeling Kit

ARES™ Alexa Fluor™ 647 DNA Labeling Kit The ARES™ Alexa Fluor™ DNA Labeling Kit provides a versatile, two-step method for labeling DNA with our Alexa Fluor™ dyes. In the first step, an amine-modified nucleotide is incorporated into DNA using conventional enzymatic labeling methods. In the second step, the amine-modified DNA is chemically labeled using our proprietary amine-reactive Alexa Fluor™ 647 dye. The labeled probes can be used for fluorescencein situhybridization (FISH) and microarray techniques. We offer ARES™ Alexa Fluor™ DNA Labeling Kits in five fluorescent colors, and each kit provides sufficient reagents for 5 to 10 labeling reactions of 1 to 5 μg DNA each.
ARES™ Alexa Fluor™ Labeling Kit Specifications:
• Dye (Ex/Em): Alexa Fluor™ 647 (650/670 nm)
• Achieves more uniform, consistent labeling than techniques for enzymatic incorporation of labeled nucleotides
• Typically produces one dye per 12–20 bases
• Optimal for FISH and microarraysMore Uniform Labeling With ARES™ Labeling KitsARES™ Alexa Fluor™ Labeling Kits employ a two-step labeling technology—nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor™ dyes. This method achieves uniformity and consistency of labeling that is difficult to obtain with conventional enzymatic incorporation of labeled nucleotides.
We also offer this two-step labeling technology in ourFISH Tag™ DNAandFISH Tag™ RNA Kits, which provide a complete workflow solution for FISH applications, including all of the reagents for probe synthesis, labeling, purification and an anti-fade reagent to help protect the signal during fluorescence microscopy.
More Options for Nucleic Acid LabelingTo review various options for nucleic acid labeling (including DNA and RNA FISH), reviewLabeling Oligonucleotides and Nucleic Acids—Section 8.
2in the Molecular Probes™ Handbook.
For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.
Includes Label or Dye: Yes
Labeling Method: Indirect Labeling
Product Line: ARES™, Alexa Fluor™
Product Type: DNA Labeling Kit
Quantity: 1 kit
Shipping Condition: Room Temperature
Detection Method: Fluorescence
Final Product Type: Probes (Labeled DNA), cDNA (Labeled)
Format: Kit
Labeling Target: DNA (General), cDNA
Label or Dye: Alexa Fluor 647
Unit Size: Each