Thermo Fisher, A24372, GeneArt™ Genomic Cleavage Detection Kit

GeneArt™ Genomic Cleavage Detection Kit The GeneArt Genomic Cleavage Detection Kit is a fast, T7 endonuclease I based method to quantify how well your genome editing protocol causes insertions and deletions (indels) in the genome of your cell line. This is the quickest way to quantify and validate the best CRISPR-Cas9 gRNA, or the best TAL effector nuclease, for your cell engineering experiment. The GeneArt Genomic Cleavage Detection Kit provides a convenient, quick, and complete solution.
Using this T7 endonuclease I (T7EI) based method, you can quickly and confidently measure your on-target genome editing efficiency generated by non-homologous end joining (NHEJ) activity. The advantages of the GeneArt Genomic Cleavage Detection Kit are:
•Minimal hands-on time— PCR amplify from cell lysate, no DNA purification required
•Short protocol— four hours from cell harvest to quantified results
•Just add PCR primers— everything needed in one box. No separate PCR master mixes or DNA extraction kits to buy.
•Quantify editing efficiency directly from your gel— gel band density directly correlates to on-target indel formationObtain results the same day without waiting for sequencing results or performing complicated sequencing analysis.
Format: Kit
No. of Reactions: 20 Reactions
Polymerase: Taq Polymerase
Product Type: Genomic Cleavage Detection Kit
Quantity: 20 reactions
Recognition Site: INDEL
Sufficient For: 20 Reactions
Technique: CRISPR-Cas9, TAL Effector Nuclease
Detection Method: Primer-probe
Form: Liquid
Reaction Speed: Fast
Unit Size: Each