Thermo Fisher, A26808, Precision ID Identity and Library Kit Bundle

Precision ID Identity and Library Kit Bundle The Applied Biosystems™ Precision ID Identity Panel and Library Kit Bundle is packaged for easy ordering and preparation of amplicon libraries using the Precision ID Identity Panel (Cat. No. A25643) and the Precision ID Library Kit (Cat. No. A26435) for sequencing on Ion Torrent sequencing systems. The Precision ID Identity Panel of multiplexed SNPs provides unique identification of degraded or trace samples with discrimination similar to the STR genotype match probabilities used by forensic analysts (between 1x10–31and 6x10–35).
Simple protocol
• 96 well plate-based workflow enables easy sample management
• No library amplification with qPCR quantificationOptimized digestion reagent
• Cleaner digest allows for more efficient downstream sequencingScalable multiplex PCR reactionsThe Precision ID Identity Panel is built on Ion AmpliSeq™ chemistry which enables scalable multiplex PCR reactions for all 124 markers in the panel using just 1 ng of starting DNA, with superior coverage uniformity and specificity (acceptable performance has also been observed with sub-nanogram input quantities as low as 180 pg). In addition, the primers contain proprietary modifications that enable removal of primer sequences during library preparation, for efficient target assessment during sequencing. Multiple primer pools can be used to create overlapping amplicons that enable complete coverage of large targets.
Learn more about other Precision ID panels >The included Precision ID Library Kit includes reagents for generating amplicons with Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using the Ion Xpress™ Barcode Adapters 1-96 Kit, and the IonCode™ Barcode Adapters 1-384 Kit. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize sequencing run time and cost and allow for accurate sample-to-sample comparisons.
Easy and flexible protocolThe resulting DNA libraries are ready for downstream template preparation for clonal amplification on Ion Sphere™ particles using the automated Ion OneTouch™ 2 System. The intuitive Torrent Suite Software and HID SNP Genotyper plug-in enable you to go from extracted DNA to ancestry-informative genotypes in less than two days*.* Using the Applied Biosystems™ Precision ID NGS System for Human IdentificationFormat: Kit
Sample Type: DNA
Sequencing Type: Genome and DNA Sequencing
Final Product Type: Genomic DNA (Targeted Regions)
For Use With (Equipment): Ion GeneStudio S5 System, Ion PGM™ System, Ion S5™ XL System, Ion OneTouch™ 2 System, Ion Chef™ System
For Use With (Application): Sequencing
Libraries: Targeted Sequencing Library
No. of Reactions: 96
Product Line: AmpliSeq™
Product Type: Precision ID Identity and Library Kit
Quantity: 96 Reactions
Shipping Condition: Dry Ice
Species: Human
Workflow Step: Library Generation
Unit Size: Each