Thermo Fisher, A30629, Alexa Fluor™ 488 Hydroxylamine

Alexa Fluor™ 488 Hydroxylamine Alexa Fluor™ 488 Hydroxylamine is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins. Alexa Fluor™ 488 is a bright, green fluorescent dye with excitation ideally suited to the 488 nn laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 488 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Alexa Fluor™ 488 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.
Detailed information about this AlexaFluor™ Hydroxylamine:Fluorophore label:Alexa Fluor™ 488 dyeReactive group:HydroxylamineReactivity:Aldehydes or ketonesEx/Em of the conjugate:494/518 nmExtinction coefficient:77,000 cm-1M-1Spectrally similar dyes:FITC, GFPMolecular weight:895.
07Cell Tracking and Tracing ApplicationsAlexa Fluor™ hydrazides and Hydroxylamines are useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores. They are easily loaded into cells by microinjection, infusion from patch pipette, or uptake induced by our Influx™ Pinocytic Cell-Loading Reagent (Cat. No. I14402).
Glycoprotein and Polysaccharide Labeling ApplicationsThe Alexa Fluor™ hydrazides and Hydroxylamines are reactive molecules that can be used to add a fluorescent label to biomolecules containing aldehydes or ketones. Aldehydes and ketones can be introduced into polysaccharides and glycoproteins by periodate-mediated oxidation of vicinal diols. Galactose oxidase can also be used to oxidize terminal galactose residues of glycoproteins to aldehydes.
Hydrazide vs HydroxylamineHydrazine derivatives react with ketones and aldehydes to yield relatively stable hydrazones. Hydroxylamine derivatives (aminooxy compounds) react with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. Both hydrazones and oximes can be reduced with sodium borohydride (NaBH4) to further increase the stability of the linkage.
Learn More About Protein and Antibody LabelingWe offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See our Antibody Labeling kits or use our Labeling Chemistry Selection Tool for other choices. To learn more about our labeling kits, read Kits for Labeling Proteins and Nucleic Acids—Section 1.
2 in The Molecular Probes™ Handbook.
We'll Make a Custom Conjugate for YouIf you can't find what you're looking for in our online catalog, we'll prepare a custom antibody or protein conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.
Chemical Reactivity: Carboxylic Acid, Ketone, Aldehyde
Excitation: 494 nm
Label or Dye: Alexa Fluor™ 488
Product Type: Hydroxylamine
Quantity: 1 mg
Reactive Moiety: Amine, Hydroxylamine
Shipping Condition: Room Temperature
Label Type: Alexa Fluor
Product Line: Alexa Fluor™
Unit Size: Each