Thermo Fisher, A30632, Alexa Fluor™ 647 Hydroxylamine

Alexa Fluor™ 647 Hydroxylamine Alexa Fluor™ 647 Hydroxlamine is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins. Alexa Fluor™ 647 is a bright, far red fluorescent dye with excitation ideally suited to the 633 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 647 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Alexa Fluor™ 647 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection (learn more).
Detailed information about this AlexaFluor™ hydroxlamine:
• Fluorophore label : Alexa Fluor™ 647 dye
• Reactive group: hydroxlamine
• Reactivity: Aldehydes or ketones
• Ex/Em of the conjugate: 651/672 nm
• Extinction coefficient: 250,000 cm-1M-1
• Spectrally similar dyes: APC, Cy5
• Molecular weight: ∼1,200Cell Tracking and Tracing ApplicationsAlexa Fluor™ hydrazides and hydroxlamines are useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores. They are easily loaded into cells by microinjection, infusion from patch pipette, or uptake induced by ourInflux™ Pinocytic Cell-Loading Reagent.
Learn more about cell tracking and tracing.
Glycoprotein and Polysaccharide Labeling ApplicationsThe Alexa Fluor™ hydrazides and hydroxlamines are reactive molecules that can be used to add a fluorescent label to biomolecules containing aldehydes or ketones. Aldehydes and ketones can be introduced into polysaccharides and glycoproteins by periodate-mediated oxidation of vicinal diols. Galactose oxidase can also be used to oxidize terminal galactose residues of glycoproteins to aldehydes.
Hydrazide vs HydroxylamineHydrazine derivatives react with ketones and aldehydes to yield relatively stable hydrazones. Hydroxylamine derivatives (aminooxy compounds) react with aldehydes and ketones to yield oximes. Oximes are superior to hydrazones with respect to hydrolytic stability. Both hydrazones and oximes can be reduced with sodium borohydride (NaBH4) to further increase the stability of the linkage.
Learn More About Protein and Antibody LabelingWe offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See ourAntibody Labeling kitsor use ourLabeling Chemistry Selection Toolfor other choices. To learn more about our labeling kits, readKits for Labeling Proteins and Nucleic Acids—Section 1.
2in The Molecular Probes™ Handbook.
We’ll Make a Custom Conjugate for YouIf you can’t find what you’re looking for in our online catalog, we’ll prepare a custom antibody or protein conjugate for you. Ourcustom conjugation serviceis efficient and confidential, and we stand by the quality of our work. We are ISO 13485:2000 certified.
Chemical Reactivity: Carboxylic Acid, Ketone, Aldehyde
Emission: 672 nm
Excitation: 651 nm
Label or Dye: Alexa Fluor™ 647
Product Type: Hydroxylamine
Quantity: 1 mg
Reactive Moiety: Amine, Hydroxylamine
Shipping Condition: Room Temperature
Label Type: Alexa Fluor
Product Line: Alexa Fluor™
Unit Size: Each