Product Description
TaqMan™ Advanced miRNA Human Endogenous Controls 96-well Plate, standard The Applied Biosystems TaqMan Advanced Human Endogenous Controls 96-Well Plate, Standard, provides the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains 1 plate than can be used to evaluate 30 potential endogenous control miRNAs for up to 3 samples. Two exogenous (non-human) controls are included for normalization of sample input.
Features of the TaqMan Advanced miRNA Human Endogenous Controls 96-Well Plate include:
•Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
•Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
•Updated content—assay content updated for mirBase v.
21
•Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
•Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific's industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA familiesProduct details:Number of plates:1Number of unique miRNAs (not including exogenous controls):30Exogenous controls:cel-miR-39-3p, ath-miR159aCompatible with the following Applied Biosystems qPCR instruments:Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k FlexRecommended master mix:TaqMan Fast Advanced Master MixNotes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
For Use With (Equipment): 7500 System, 7900 HT System, QuantStudio™ 12 k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, ViiA™ 7 System, StepOnePlus™ System
Format: 96-Well Plate
Product Line: TaqMan™
Product Type: Endogenous Control 96-Well Plate
Quantity: 1 plate
Sample Type: Liquid Samples (e.
g. Serum), Tissue, Cells, FFPE & Fixed Samples, Blood
Shipping Condition: Room Temperature
Volume: 2 μL
Detection Method: Primer-probe
For Use With (Application): Microarray Analysis
GC-Rich PCR Performance: High
PCR Method: 2-step RT-qPCR
Reaction Speed: Standard
Unit Size: Each
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