Thermo Fisher, A36806, Pierce™ 3x DYKDDDDK Peptide

25 mg Pierce 3X DYKDDDDK Peptide is a lyophilized peptide with the sequence Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (MDYKDHDGDYKDHDIDYKDDDDK). It has a molecular weight of 2861.
9 g/mol. For use, reconstitute to 1.
5 mg/mL in PBS.
This tricosa-peptide can be used to competitively elute DYKDDDDK-tagged fusion proteins from immobilized anti-DYKDDDDK affinity supports, especially when a DYKDDDDK-tagged protein is sensitive to low pH. At a 1.
5 mg/mL concentration, complete release of tagged protein can be achieved in 5–10 minutes at ambient temperature.
Note:Pierce 3X DYKDDDDK Peptide may interfere with protein determination assays and absorbance at 280 nm. However, the peptide can be removed from the eluate by desalting (e.
g., with Zeba Spin Desalting Columns, Cat. No. 89891).
For Use With (Application): Protein Purification and Isolation
Form: Lyophilized Powder
Format: Lyophilized Powder
Product Line: Pierce™
Product Type: Peptides
Protein Form: Recombinant
Quantity: 5 x 5 mg
Shipping Condition: Wet Ice
Matrix: Affinity Resin
Unit Size: Each