Thermo Fisher, A45916, SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto

SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto This substrate bundle contains SuperSignal West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.
SuperSignal West Femto Maximum Sensitivity SubstrateThermo Scientific SuperSignal West Femto Maximum Sensitivity Substrate is a high-sensitivity enhanced chemiluminescent (ECL) substrate for low femtogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is optimized to detect low abundant and/or difficult-to-detect targets.
• Sensitivity: low-femtogram (mid-zeptomole)
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to eight hours
• Recommended primary antibody concentration: 1:5,000–1:100,000 dilution (10–200 ng/mL)
• Recommended secondary antibody concentration: 1:100,000–1:500,000 dilution (2–10 ng/mL)View full product information ›SuperSignal West Atto Ultimate Sensitivity Chemiluminescent SubstrateThermo Scientific SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is designed for detection of very low-abundant targets or the maximum levels of sensitivity required when using precious samples or costly antibodies.
• Sensitivity: low-femtogram to high-attogram
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to four hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.
2–1.
0 μg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)View full product information ›Sensitivity: Low-femtogram (mid-zeptomole)
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
Sufficient For: 12 Mini-Gel Blots, 1000 sq. cm of Membrane; 2 Mini-Gel Blots, 200 sq. cm of Membrane
Detection Method: Chemiluminescence
For Use With (Application): Western Blot
Form: Liquid
Membrane Compatibility: Nitrocellulose, PVDF
Product Line: SuperSignal™
Product Type: Substrate
Quantity: 100 mL + 20 mL bundle
Substrate: Horseradish Peroxidase
Unit Size: Each