Thermo Fisher, A45917, SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS

SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS This substrate bundle contains SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.
SuperSignal West Atto Ultimate Sensitivity Chemiluminescent SubstrateThermo Scientific SuperSignal West Atto Ultimate Sensitivity Chemiluminescent Substrate is an ultra-sensitive enhanced chemiluminescent (ECL) substrate for high attogram protein level detection by western blot analysis with horseradish peroxidase (HRP) enzyme. It is designed for detection of very low-abundant targets or the maximum levels of sensitivity required when using precious samples or costly antibodies.
• Sensitivity: low-femtogram to high-attogram
• Stability: eight hours for working solution, one year for kit at 4°C
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: up to four hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.
2–1.
0 μg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 dilution (4–10 ng/mL)View full product information ›SuperSignal West Pico PLUS Chemiluminescent SubstrateThermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate enables picogram to high femtogram-level protein detection by western blot analysis. It is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for routine western blotting applications.
• Sensitivity: picogram to femtogram
• Stability: eight hours for working solution; one year for kit at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 6 to 24 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.
2–1.
0 μg/mL)
• Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)View full product information ›Sensitivity: Picogram to femtogram, low-femtogram to high-attogram
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
Sufficient For: 12 Mini-Gel Blots, 1000 sq. cm of Membrane; 2 Mini-Gel Blots, 200 sq. cm of Membrane
Detection Method: Chemiluminescence
For Use With (Application): Western Blot
Form: Liquid
Membrane Compatibility: Nitrocellulose, PVDF
Product Line: SuperSignal™
Product Type: Substrate
Quantity: 100 mL + 20 mL bundle
Substrate: Horseradish Peroxidase
Unit Size: Each