Thermo Fisher, A47929, TaqMan™ SCID/SMA Assay

20,000 Reactions The Applied Biosystems TaqMan SCID/SMA Assay is a multiplex real-time PCR assay that detects theSMN1gene and TREC, as well as the RNase P gene as an internal genomic control. For an assay that also detects KREC, please see the TaqMAN SCID/SMA Plus Assay (Cat. No. A48566).
Features of the TaqMan SCID/SMA Assay include:
• Optimized for SCID and SMA
• Direct extraction from DBS samples for reduced hands-on time and cost
• Compatible with 96- or 384-reaction protocols
• High-quality results for 96 samples within 55 min using the fast cycling protocol
• Minimal liquid-dispensing steps for easy automationComplete solution from DBS to resultThe TaqMan SCID/SMA Assay detectsSMN1, TREC, as well as the RNase P gene as an internal genomic control. The assay solution contains a mixture of primers and probes for each target plus anSMN2blocker. From DBS to result, less than 40 min of hands-on time and only four reagent-dispensing steps are required for 96-well plates. One additional pipetting transfer step from DBS lysate extraction plate to qPCR plate is needed. A real-time PCR instrument with at least five filters is required to detect the reporter dyes plus the passive reference dye.
The TaqMan SCID/SMA Assay is a multiplex real-time PCR assay that detects exon 7 ofSMN1and TREC. The assay includes the RNase P gene as an internal amplification control, verifying successful sample extraction. The assay is optimized for high specificity toSMN1, eliminating cross-detection ofSMN2.
Detection Method: Primer-probe
For Use With (Equipment): QuantStudio™ 12 k Flex, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, QuantStudio™ Dx System (in RUO Mode)
Product Line: TaqMan™
Quantity: 20,000 reactions
Shipping Condition: Dry Ice
No. of Reactions: 20,000 Reactions
Sample Type: DNA (Genomic), cDNA
Unit Size: Each