Thermo Fisher, A51802, SuperScript™ IV RT-LAMP Master Mix

400 Reactions Invitrogen SuperScript IV RT-LAMP Master Mix is a ready-to-use solution optimized for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions. With this convenient master mix formulation, you can perform isothermal amplification from both RNA or DNA targets in a single tube format.
Benefits of SuperScript IV RT-LAMP Master Mix
• RNA and DNA target detection using Bst DNA polymerase for isothermal amplification in as little as five minutes
• Robust and specific cDNA synthesis using SuperScript IV Reverse Transcriptase
• Enhanced specificity and sensitivity with buffer formulation and RNaseOUT recombinant ribonuclease inhibitor
• Compatible with real-time and endpoint detection—includes SYTO 9 dye for real-time detection
• Flexible design provides several options for evaluating results, including real-time and endpoint detection methodsSuperScript IV RT-LAMP Master Mix includes an evolved Bst DNA polymerase mutant with a fast reaction speed and increased sensitivity and tolerance to inhibitors, as well as SuperScript IV Reverse Transcriptase with increased processivity, thermostability, and inhibitor resistance. To prevent RNA degradation by carry-over RNases, the master mix includes the potent, noncompetitive RNaseOUT Recombinant Ribonuclease Inhibitor. Our proprietary reaction buffer composition is optimized to increase specificity by delaying no-template control (NTC) amplification time.
Concentration: 2X
Detection Method: Real-time and End-point Detection
Includes Label or Dye: SYTO 9 Green Fluorescent Nucleic Acid Stain
No. of Reactions: 400 Reactions
Optimal Reaction Temperature: 65°C
Reaction Time: 5 to 15 minutes
Reverse Transcriptase: SuperScript IV
Shipping Condition: Dry Ice
For Use With (Application): LAMP, RT-LAMP
Polymerase: Bst DNA Polymerase
Product Type: Master Mix
Quantity: 400 reactions, 4 x 1.
25 mL
Unit Size: Each