Product Description
TrueTag™ Donor DNA Kit, GFP stem The Invitrogen TrueTag Donor DNA Kit, GFP stem, provides a rapid solution to drive high-efficiency tagging (up to 100% edited cells) of silent or differentially expressed genes with transfection-ready donor DNA.
Benefits of the TrueTag Donor DNA stem kits include:
• One-step PCR amplification to generate donor templates—no cloning required
• Generate transfection-ready DNA donors in hours instead of days
• Save up to three months with enrichment compared to single cell isolation and characterization
• Enrich up to 100% GFP-tagged cellsThe TrueTag Donor DNA Kit, GFP stem, provides everything you need to produce a high-quality DNA donor to tag your gene with a selection marker and GFP—simply provide two locus-specific primers to add homology arms and PCR-amplify the donor. Each kit is designed to enable you to:
• Enrich tagged cells even when the target protein is not being expressed
• Choose between a fusion or co-expressed fluorescent tag
• Choose from puromycin or blasticidin selection markers—whatever best suits your cell model
• Easily remove antibiotic resistance markers with Cre/Lox following cell enrichmentIncluded in the kit
• Four linear donor templates: C-terminus/puromycin resistance + 2A, C-terminus/blasticidin resistance + 2A, C-terminus/puromycin resistance, C-terminus/blasticidin resistance
• Phusion Flash High-Fidelity PCR Master Mix
• PCR cleanup columns and buffers
• Positive control primers to tag human glial fibrillary acidic protein (GFAP)TrueDesign Genome Editor software to rapidly design gene-specific reagentsTarget-specific gene editing reagents are required for proper knock-in of your fluorescent tag. Identify your target gene transcript of interest and theTrueDesign Genome Editorweb-based software does the work for you:1. Displays your target gene transcript2. Designates your insertion site3. Designs and enables easy ordering of all required components not included in the TrueTag kit:
• CRISPR guide RNA or TALENs designed adjacent to N- or C-terminus
• Oligos to PCR-amplify your donor and add appropriate homology arms
• Sequencing primers for assessment of CRISPR-Cas9 cleavage efficiency
• Verification primers to validate proper insertion of the TrueTag DNA DonorAlso required and available for purchase separately or as part of TrueDesign workflow:
•TrueCut Cas9 Protein v2,TrueCut HiFi Cas9 Protein, orGeneArt CRISPR mRNA
•CRISPRMAX Transfection ReagentorMessengerMAX Transfection ReagentFor Use With (Application): Cellular Imaging
Quantity: 10 Reactions
Unit Size: Each
Order Guidelines
1. Price & Stock Available on Request. Click to send email to: service@iright.com
2. Please DO NOT make payment before confirmtaion.
3. Minimum order value of $1,000 USD required.
4. 100% prepayment required.
Collaboration
Tony Tang
Email: Tony.Tang@iright.com
Mobile/WhatsApp/Wechat: +86-17717886924