Thermo Fisher, A53815, TrueTag™ Knockout Enrichment Donor DNA Kit

TrueTag™ Knockout Enrichment Donor DNA Kit The Invitrogen TrueTag Knockout Enrichment Donor DNA Kit provides a rapid solution to drive high-efficiency gene knockout and enrichment (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.
Benefits of the TrueTag Knockout Enrichment Donor DNA Kit include:
• Easily design and order all necessary components to generate a knockout cell line
• Save up to 3 months with enrichment compared to single cell isolation and characterization
• Leverage a selection marker to obtain up to 100% edited cellsThe TrueTag Knockout Enrichment Donor DNA Kit provides everything you need to achieve functional gene knockout and enrich the edited cells. The donor produced will insert 3X STOP codons, a selection marker, and a fluorescent tag—simply provide two locus-specific primers to add homology arms and PCR-amplify the donor. Each kit is designed to enable you to:
• Insert 3X STOP codons to knock out gene function
• Tag each allele with a different fluorophore (GFP or RFP) to verify bi-allelic knockout
• Utilize puromycin and blasticidin selection markers for bi-allelic knockout confirmation
• Easily remove antibiotic resistance markers with Cre/Lox following cell enrichmentIncluded in the kit
• Four linear donor templates: GFP/puromycin resistance, GFP/blasticidin resistance, RFP/puromycin resistance, RFP/blasticidin resistance
• Phusion Flash High-Fidelity PCR Master Mix
• PCR cleanup columns and buffers
• Positive control primers for knockout of humanHPRT1TrueDesign Genome Editor software to rapidly design gene-specific reagentsTarget-specific gene editing reagents are required for proper knock-in of your fluorescent tag. Identify your target gene transcript of interest and theTrueDesign Genome Editorweb-based software does the work for you:1. Displays your target gene transcript2. Designates your insertion site3. Designs and enables easy ordering of all required components not included in the TrueTag kit:
• CRISPR guide RNA or TALENs designed adjacent to N- or C-terminus
• Oligos to PCR-amplify your donor and add appropriate homology arms
• Sequencing primers for assessment of CRISPR-Cas9 cleavage efficiency
• Verification primers to validate proper insertion of the TrueTag DNA DonorAlso required and available for purchase separately or as part of TrueDesign workflow:
•TrueCut Cas9 Protein v2,TrueCut HiFi Cas9 Protein, orGeneArt CRISPR mRNA
•CRISPRMAX Transfection ReagentorMessengerMAX Transfection ReagentFor Use With (Application): Gene Functional Analysis
Quantity: 10 Reactions
Unit Size: Each