Thermo Fisher, A66428, AgriSeq™ Genomic DNA Extraction Kit

AgriSeq™ Genomic DNA Extraction Kit The AgriSeq Genomic DNA Extraction Kit enables generation of PCR-ready genomic DNA for next-generation sequencing and other applications from a variety of aquaculture and plant tissues in less than 10 minutes. No bead beating or mechanical homogenization of tissues is required. After DNA extraction, NGS amplicon library prep can be carried out using the AgriSeq HTS Library kit workflow.
Features of the AgriSeq Genomic DNA Extraction Kit include:Simple and fast protocol—takes less than 10 mins with few stepsUniversal—purifies genomic DNA from aquaculture and plant tissuesNo bead beading or mechanical homogenization of tissuesDoes not involve phenol extractionApplications: NGS and qPCRHow to useSimply mix your tissue sample with the AgriSeq Genomic DNA Extraction lysis solution provided. Perform direct lysis by incubating for 2–5 minutes on a thermal cycler at 95–97°C for cell lysis. After lysis lower the temperature to 25°C for 3 minutes and add the AgriSeq Genomic DNA stabilizing solution to complete the crude lysis isolation. Spin the plate down briefly in the centrifuge to separate the tissue residue and the lysate. Store crude lysate at 4°C short-term or –20°C long-term or proceed directly to PCR amplification.
Note:For NGS applications, add 1 μL of Plant RNA Isolation Aid per 10 μL total reaction volume in place of nuclease-free water. For example, in a 25 μL reaction volume, replace 2.
5 μL of water with 2.
5 μL RNA Isolation Aid.
For Use With (Application): Genomic DNA extraction using a simple direct cell lysis protocol for downstream NGS and qPCR applications
No. of Reactions: 9600 Reactions
Nucleic Acid Type: DNA
Optimal Reaction Temperature: 95°C to 97°C (Lysis initiation), 25°C (Lysis incubation)
PCR Method: Multiplex Amplicon PCR
Plate Compatibility: MicroAmp™ EnduraPlate™ Optical 96-well Clear Reaction Plates
Plate Type: Deep-well
Product Line: AgriSeq™
Product Type: Genomic DNA Extraction Kit
Quantity: 9600 reactions
Sample Source: Plants: leaf, seed, or tissue; Aquaculture: Fins, Plieopods
Sample Type: Leaf Punches, Seed Chips, Tissue pieces, Fin Punches, Plieopods
Shelf Life: 14 Months
Shipping Condition: Cool Gel Pack
Species: Plants, Aquaculture
Starting Material Amount: 2 x 3 mm (or 1 x 5 to 6 mm) leaf/fin punch
Workflow Step: DNA Extraction
Format: 96-well Plate
Unit Size: Each