Thermo Fisher, AM1561, mirVana™ miRNA Isolation Kit, without phenol

mirVana™ miRNA Isolation Kit, without phenol ThemirVana™ miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method. The method isolates total RNA ranging in size from kilobases down to 10-mers. Each kit contains sufficient reagents and consumables for either 40 isolations of total RNA (including small RNAs), or 20 separate large and small RNA fractions. Phenol must be purchased separately. Features of this kit:
• Efficient isolation of small RNA-containing total RNA
• Enrich for small RNA (less than 200 nt) to increase sensitivity in downstream analyses
• Simple 30 minute procedure
• Ideal for miRNA, siRNA, shRNA, and snRNA analysis
• Compatible with virtually all cell and tissue typesEfficient recovery of small RNACurrent RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix. Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and inefficiently recovers small RNAs. The latter procedure typically results in the loss of substantial amounts of small RNA. ThemirVana™ miRNA Isolation Kit uses organic extraction followed by purification on a GFF under specialized binding and wash conditions. As a result, the kit effectively recovers all RNA—from large mRNA and ribosomal RNA down to 10-mers—from virtually all cell and tissue types. ThemirVana™ miRNA Isolation Kit enables the isolation of small RNA-containing total RNA from samples consisting of 102–107cultured cells or 0.
5–250 mg tissue.
Enrich for small RNAAlthough themirVana™ miRNA Isolation Kit efficiently purifies all RNA larger than 10 nt, it also includes procedures for isolating RNA fractions, specifically enriched or depleted in small RNA species. Through the enrichment procedure included in themirVana™ miRNA Isolation Kit, RNA molecules of ∼200 nt and less can be efficiently purified away from the larger RNA species. The resulting RNA preparation is highly enriched for miRNAs, siRNAs, and/or snRNAs. Small RNA enrichment using themirVana™ miRNA Isolation Kit procedure allows more sensitive small RNA detection with less background as compared to the same assay used with total RNA.
Accessory productThemirVana™ miRNA Isolation Kitis available for those who want a complete kit that includes the Acid Phenol:Chloroform.
Elution Volume: 100 μL
Final Product Type: Small RNA
For Use With (Application): qRT-PCR, microarray analysis, Northern blotting
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Purification Time: 30 min. (After sample homogenization)
Quantity: 40 Preps
Shipping Condition: Room Temperature
Starting Material Amount: Bacteria: ≤107cellsCells: ≤107Plant: ≤250 mgTissue: ≤250 mgVirus: ≤107cellsYeast: ≤107cellsEnzymatic Reactions: varies
Yield: Cells: ≤16 μg RNA per 50,000 cellsTissue: ≤160 μg per 5 mg
Isolation Technology: Spin Column (Glass Fiber Filter), Organic Extraction
Sample Type: Bacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
Unit Size: Each