Thermo Fisher, AM4510M, siPORT™ NeoFX™ Transfection Agent with manual

Transfection Reagent, 400 μL This Ambion™ lipid-based formulation can be used to efficiently transfect adherent cells with siRNA as they are subcultured—without increased cytotoxicity. It is provided in one tube containing 1 mL.
• Versatile—works with a broad range of cell lines
• Reproducible—produces consistent results, lot-to-lot, plate-to-plate, and well-to-well
• Efficient—performs with high siRNA transfection efficiency, allowing use of low siRNA concentrations to minimize nonspecific effects
• Fast—reverse-transfects cells as they are plated, saving a dayGene silencing experiments often call for critical, but time-consuming optimization experiments. siPORT™NeoFX™Transfection Agent refines siRNA transfection protocols resulting in less optimization. From start to finish, successful gene silencing experiments can be completed in as early as 24 hours. This streamlined protocol can be adapted to a wide range of cells and experimental designs, including high-throughput applications. Reverse-Transfect Cells as They are Plated, Saving a DayJust add siPORT™NeoFX™to your diluted siRNA, incubate to form transfection complexes, add the complexes to the culture wells, and overlay with cells. This new streamlined protocol, named “neofection”, will save researchers a full day of valuable time when compared to traditional plated transfection procedures. The transfection complexes are active and stable even in the presence of serum, so there is no need to remove or replace media following transfection. Optimized for Lower siRNA Concentrations and High ReproducibilityRecent reports indicate that siRNA concentrations of 100 nM or higher can lead to nonspecific changes in gene expression (off-target effects) in mammalian cultured cells. Reducing the amount of siRNA used for transfections to 1–20 nM minimizes these nonspecific effects, while still providing extensive silencing of the target gene. siPORT™NeoFX™Transfection Agent efficiently transfects low concentrations of siRNA. Furthermore, this agent delivers high performance among multiple wells for replicate samples and between different lots of the transfection agent. Compatible with a Broad Range of Cell LinesThe agent has been used successfully to deliver siRNA into many cell types, including MCF-7 (human breast adenocarcinoma), HT-29 (human colorectal adenocarcinoma), HeLa-S3 (human cervix adenocarcinoma), HeLa (human cervix adenocarcinoma), HepG2 (human hepatocellular carcinoma), BJ (human foreskin fibroblast), A549 (human lung carcinoma), UMR106 (rat osteosarcoma), SKOv3 (human ovarian carcinoma), and SKNAS (human bone marrow neuroblastoma). Accessory Products:siPORT™AmineTransfection Agent (SKU#s AM4502 and AM4503), a polyamine agent, is also available. For testing which transfection agent is best with your system, theSilencer™ siRNA Transfection II Kit (SKU# AM1631) is recommended. It contains the two different transfection agents along with controls for transfection optimization. For Use With (Application): Transfection
High-throughput Compatibility: High-throughput Compatible
Product Line: Ambion™, siPORT™
Product Type: Transfection Reagent with Manual
Quantity: 400 μL
Serum Compatible: Yes
Cell Type: Stem Cells, Primary Cells, Mammalian
Format: 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample Type: Synthetic siRNA
Transfection Technique: Lipid-based Transfection
Unit Size: Each