Thermo Fisher, C101010, One Shot™ PIR1 Chemically Competent E. coli

One Shot™ PIR1 Chemically Competent E. coli One Shot PIR1 Chemically CompetentE. colicells are for use with vectors that contain the R6Kγ origin of replication (e.
g., pUni/V5-His-TOPO for recombination with an Echo cloning system). Thepirgene encodes the replication protein π, which is required to replicate and maintain plasmids containing the R6Kγ origin. The PIR1 strain contains a mutant allele of thepirgene that maintains the donor vector construct at ∼250 copies per cell. PIR1 cells are competent and can achieve high transformation efficiency of >1 x 109cfu/μg supercoiled plasmid.
R6Kγ allows tight control and selection and has been used in various applications including genome engineering, mutagenesis studies, bacterial artificial chromosome (BAC) construction, high-capacity extrachromosomal vector systems, and suicide plasmid construction.
One Shot PIR1 Chemically CompetentE. colicells offer:
•pirgene to maintain vectors that contain the R6Kγ ori at ∼250 copies per cell
• Transformation efficiencies of >1 x 109cfu/μg
•endA1 mutation enables increased plasmid DNA yield and quantity
•recA1 mutation reduce occurrence of unwanted recombination in cloned DNA
•hsdRfor efficient transformation of unmethylated DNA from PCR applicationsConvenient and efficient formatPIR1 Chemically CompetentE. colicells are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation, reducing freeze-thaw cycles and money wasted on unused cells.
GenotypeF–∆lac169rpoS(Am)robA1creC510hsdR514endArecA1uidA(∆MluI)::pir-116Genetic marker descriptionsFind the strain and format that fit your needsMultiple DH strains are available inchemically competentandelectrocompetent cellformats.
We offerPIR2 strainthat carries wild-typepirgene and maintain vectors at ∼15 copies per cell.
MultiShot formats are available for high throughput applications.
Explorebacterial growthmedia formats.
Antibiotic Resistance Bacterial: No
Blue/White Screening: No
Cloning Methylated DNA: No
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Preparing Unmethylated DNA: No
Product Line: One Shot™
Product Type: Chemically Competent Cells
Quantity: 11 x 50 μL/tube
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Format: Tube
Species: E. coli
Unit Size: Each