Product Description
Thermo Fisher, C10491, CellROX™ Deep Red Flow Cytometry Assay Kit The CellROX™ Deep Red Flow Cytometry Assay Kit enables the flow cytometric detection of reactive oxygen species (ROS) in live cells. The kit includes the novel, fluorogenic CellROX™ Deep Red Reagent, as well as SYTOX™ Blue dead cell stain, N-acetyl cysteine (an antioxidant, for negative control), and tert-butyl hydroperoxide solution (TBHP, an inducer of ROS).
View a selection guide for allCellROX™ Reagents and Kits.
The CellROX™ Deep Red Flow Cytometry Assay Kit features:
• Fluorogenic probe formulated for flow cytometry that is oxidized in the presence of ROS
• Multicolor compatibility—minimal overlap with fluorophores excited by other laser lines, allowing easy multiplexing with other reagents
• Simple protocol—cells can be stained in complete media or other appropriate buffer, no need for serum free mediaThe CellROX™ Deep Red Detection Reagent is cell-permeable and non-fluorescent or very weakly fluorescent while in the reduced state. Upon oxidation, the reagent exhibits a strong fluorogenic signal that has absorption/emission maxima of 644/665 nm and remains localized in the cytoplasm. When used together with the included SYTOX™ Blue Dead Cell Stain, oxidatively stressed and non-stressed cells are reliably distinguished from dead cells by flow cytometry.
Cell Type: Mammalian Cells, Eukaryotic Cells
Detection Method: Fluorescence
Dye Type: CellROX™ Deep Red Reagent
Format: Tube(s)
Quantity: 100 Assays
Shipping Condition: Wet Ice
Solubility: DMSO (Dimethylsulfoxide)
Emission: 644/665, 444/480
For Use With (Application): Flow Cytometry
For Use With (Equipment): Flow Cytometer
Product Line: CellROX
Product Type: Reagent
Unit Size: Each
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Collaboration
Tony Tang
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