Thermo Fisher, C111110, One Shot™ PIR2 Chemically Competent E. coli

One Shot™ PIR2 Chemically Competent E. coli One Shot PIR2 Chemically CompetentE. colicells are for use with vectors that contain the R6Kγ origin of replication (e.
g., pUni/V5-His-TOPO for recombination with an Echo cloning system). Thepirgene encodes the replication protein π, which is required to replicate and maintain plasmids containing the R6Kγ origin. The PIR2 strain contains the wild-typepirgene that maintains the donor at ∼15 copies per cell. This strain is recommended for maintaining constructs that express toxic genes or for libraries. PIR2 is made chemically competent and can achieve high transformation efficiency of >1 x 108cfu/μg supercoiled plasmid.
R6Kγ allows tight control and selection and has been used in various applications including genome engineering, mutagenesis studies, bacterial artificial chromosome (BAC) construction, high-capacity extrachromosomal vector systems, and suicide plasmid construction.
One Shot PIR2 Chemically CompetentE. colicells offer:
• Wild-typepirgene to maintain vectors that contain the R6Kγ ori at ∼15 copies per cell
• Transformation efficiencies of >1 x 108cfu/μg
•endA1 mutation that enables increased plasmid DNA yield and quantity
•recA1 mutation that reduces the occurrence of unwanted recombination in cloned DNA
•hsdRfor efficient transformation of unmethylated DNA from PCR applicationsConvenient and efficient formatPIR2 Chemically CompetentE. colicells are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation, reducing freeze-thaw cycles and money wasted on unused cells.
GenotypeF–∆lac169rpoS(am)robA1creC510hsdR514endArecA1uidA(∆MluI)::pirGenetic marker descriptionsFind the strain and format that fit your needsMultiple DH strains are available inchemically competentandelectrocompetent cellformats.
We offer thePIR1 strainthat carries mutated pir gene and maintain vectors at ∼250 copies per cell.
MultiShot formatsare available for high throughput applications.
Explorebacterial growthmedia formats.
Antibiotic Resistance Bacterial: No
Blue/White Screening: No
Cloning Methylated DNA: No
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Preparing Unmethylated DNA: No
Product Line: One Shot™
Product Type: Chemically Competent Cells
Quantity: 11 x 50 μL/tube
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Format: Tube
Species: E. coli
Unit Size: Each