BRAND / VENDOR: Thermo Fisher

Thermo Fisher, C303003, One Shot™ TOP10F' Chemically Competent E. coli

CATALOG NUMBER: C303003

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Product Description

21 x 50 μL One Shot TOP10F´ Chemically CompetentE. coliare identical to TOP10 cells, with the addition of an F´ episome. The F´ episome carries a tetracycline resistance gene and allows isolation of single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication. In addition, the F´ episome carries thelacIq repressor for inducible expression fromtrc,tac, andlacpromoters using IPTG.
TOP10F´ competent cells are provided at a transformation efficiency of >1 x 109cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.
As a derivative of TOP10, TOP10F´ Chemically CompetentE. colicells carry mutations in the methylation-dependent restriction system (mcrA,mcrBC, andmrr) allowing them to clone both prokaryotic and eukaryotic genomic DNA and provide efficient plasmid rescue from eukaryotic genomes. TOP10F´ cells also carryrecA1 andendA1 mutations that increase insert stability and improve the quality of plasmid DNA prepared from minipreps.
One Shot TOP10F´ Chemically CompetentE. colicells offer:
• Transformation efficiencies of >1 x 109cfu/μg
• Stable F´ episome that allows isolation of ssDNA and carrieslacIq repressor for inducible expression fromtrc,tac, andlacpromoters
•hsdR for efficient transformation of unmethylated DNA from PCR amplifications
•mcrA for efficient transformation of methylated DNA from genomic preparations
•lacZΔM15 for blue/white color screening of recombinant clones on plates containing X-Gal or Bluo-Gal
•endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
•recA1 for reduced occurrence of nonspecific recombination in cloned DNANote:IPTG induction is required for blue-white color screening.
Easy-to-use One Shot formatTOP10 F´ Chemically CompetentE. colicells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, saving time and preventing contamination.
GenotypeF´{lacIq,Tn10(TetR)}mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74recA1araD139 Δ(ara-leu)7697galUgalKrpsL(StrR)endA1nupGFind the strains and formats that fit your needsDH strains are available inchemically competentandelectrocompetent cellformats.
For high throughput applications there are a variety of TOP10 strains inMultiShot formats.
For ssDNA production try strains such asTOP10F´or electrocompetentDH12S.
Explorebacterial growthmedia formats.
Antibiotic Resistance Bacterial: Yes (Streptamycin, Tetracycline)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: Yes
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Preparing Unmethylated DNA: No
Product Line: One Shot™
Product Type: Chemically Competent Cells
Quantity: 21 x 50 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Format: Tube
Promoter: Trc, Tac, Lac
Species: E. coli (K12)
Unit Size: Each

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