Thermo Fisher, C3239, Cascade Blue™ hydrazide, Trilithium Salt

Cascade Blue™ hydrazide, Trilithium Salt The cell-impermeant, fixable, polar tracerCascade Blue™ hydrazidecan be used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. This water-soluble tracer can be introduced into cells by whole-cell patch clamping, iontophoresis, osmotic lysis of pinocytic vesicles or comparable methods. The primary aliphatic amine of Cascade Blue™ hydrazide can also be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be reduced to a generate stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3). Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC (E2247).
Chemical Reactivity: Carboxylic Acid, Ketone, Aldehyde
Label or Dye: Cascade Blue™
Product Type: Hydrazide
Quantity: 10 mg
Reactive Moiety: Amine, Hydrazide
Shipping Condition: Room Temperature
Label Type: Classic Dyes
Product Line: CASCADE BLUE™
Unit Size: Each