Thermo Fisher, C404050, One Shot™ TOP10 Electrocomp™ E. coli

11 x 50 μL One Shot TOP10 ElectrocompE. colicells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids. TOP10 ElectrocompE. colicells are transformed via electroporation and achieve higher transformation efficiencies than chemically competent cells. They can therefore be used in more challenging applications, including cloning low amounts of DNA or cDNA and gDNA library construction.
TOP10E. colicells are genetically similar to the DH10B strain but have been reported to be more resilient to stress conditions like osmotic shock and acidic pH stress. These cells carry mutations in the methylation-dependent restriction system (mcrA,mcrBC, andmrr) allowing them to clone both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. Similar to other DH strains, TOP10 has thelacZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal.
One Shot TOP10 ElectrocompE. colicells offer:
• Transformation efficiencies of >1 x 1010cfu/μg
•hsdR for efficient transformation of unmethylated DNA from PCR amplifications
•mcrA for efficient transformation of methylated DNA from genomic preparations
•lacZΔM15 for blue/white color screening of recombinant clones
•endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
•recA1 for reduced occurrence of nonspecific recombination in cloned DNAEasy-to-use One Shot formatTOP10 Chemically CompetentE. colicells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and prevent contamination.
GenotypeF–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74recA1araD139 Δ(ara-leu)7697galUgalK λ–rpsL(StrR)endA1nupGFind the strain and format that fit your needsWe offer other DH strains inchemically competentandelectrocompetent cellformats.
Electroporation cuvettes with0.
1 cm,0.
2 cm, and0.
4 cmgaps are available.
The TOP10 strain is available in severalMultiShot formats for high throughput applications.
Explorebacterial growthmedia formats.
Note:TOP10 cells do not require IPTG to induce expression from thelacpromoter. A high-voltage electroporation apparatus is required.
Antibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: No
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Plasmid: May be used for plasmids >20 kb
Preparing Unmethylated DNA: No
Product Line: One Shot™
Product Type: Electrocompetent Cells
Quantity: 11 x 50 μL
Reduces Recombination: Yes (recA13)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes (Streptomycin)
Transformation Efficiency Level: High Efficiency (>1 x 1010cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each