Thermo Fisher, C4081201, MultiShot™ FlexPlate TOP10 Competent Cells

MultiShot™ FlexPlate TOP10 Competent Cells MultiShot FlexPlate TOP10 chemically competentE. colicells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments to allow for flexibility in transformation throughput.
Versatile TOP10 cloning capabilitiesMultiShot FlexPlate TOP10 Competent Cells are the laboratory workhorse for chemical transformations as they are compatible with a broad spectrum of DNA types for transformation. The TOP10 strain is ideal for high-efficiency cloning and plasmid propagation, achieving transformation efficiency of >1 x 108cfu/μg with control plasmid DNA in FlexPlate format. It allows stable replication of high-copy number plasmids and is the same competent cell strain that come with many of our cloning kits.
TOP10E. colicells are genetically similar to the DH10B strain, but has been reported to be more resilient to stress conditions (osmotic shock and acidic pH stress). These cells carry mutations in the methylation-dependent restriction system (mcrA,mcrBC, andmrr), allowing the cloning of both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. In addition,recA1 andendA1 mutations increase insert stability and improve the quality of plasmid DNA prepared from minipreps.
MultiShot FlexPlate TOP10 Competent Cells offer:
• Transformation efficiencies of >1 x 108cfu/μg
•hsdR for efficient transformation of unmethylated DNA from PCR amplifications
•mcrA for efficient transformation of methylated DNA from genomic preparations
•lacZΔM15 for blue/white color screening of recombinant clones on plates containing either X-Gal or Bluo-Gal
•endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
•recA1 for reduced occurrence of non-specific recombination in cloned DNANote:TOP10 cells do not require IPTG to induce expression from thelacpromoter.
Increase transformation productivity with MultiShot FlexPlate formatThe FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.
Key benefits of the MultiShot FlexPlate format
•Increased throughput—compatible with automated liquid handling platforms
•Flexible—transform in increments of 8 wells to entire 96-well plate
•Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformationGenotypeF–mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74recA1araD139 Δ(ara-leu)7697galUgalK λ–rpsL(StrR)endA1nupGFind the strain and format that you needWe offer many otherE. colistrains and formats ofchemically competent cellsandelectrocompetent cellsto help meet specific transformation needs. For high-throughput transformation, choose from our collection ofMultiShot formatted comp cellsoremail usfor custom formatting options.
Bacterial growthmedia is also available in various formats.
Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: No
Contains F' Episome: No
High-throughput Compatibility: High
Improves Plasmid Quality: Yes (endA1)
Plasmid: High Copy Plasmid
Preparing Unmethylated DNA: No
Product Line: Multishot™
Product Type: Chemically Competent Cells
Quantity: 1 x 96 well plate (20 μl/well)
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Format: 96-well Plate
Species: E. coli (K12)
Unit Size: Each