Thermo Fisher, C4481201, MultiShot™ FlexPlate DH5α T1R Competent Cells

MultiShot™ FlexPlate DH5α T1R Competent Cells MultiShot FlexPlate DH5α T1RChemically CompetentE. colicells are cloning competent cells derived from a well-known, versatile DH5α strain that can be used in many everyday cloning applications. These cells are pre-aliquoted in a 96-well PCR plate to increase transformation productivity, and the FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.
All of our T1Rstrains have thetonA (also known asfhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyseE. colihosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries.
Reliable DH5α T1Rcloning capabilitiesMultiShot FlexPlate DH5α T1RcompetentE. colicells are able to achieve transformation efficiencies of >1 x 108cfu/μg control DNA. This strain is recommended for transformations of cloned unmethylated DNA, routine cloning of genes into large plasmids, and is suitable for difficult clone constructions, such as blunt-end ligations or cloning using limited amount of DNA.
The DH5α T1Rstrain carries thehsdR mutation to prevent restriction of foreign DNA and theendA1 mutation that increases the yield of plasmid DNA prepared using miniprep protocols. ThelacZΔM15 marker provides α-complementation of the ß-galactosidase gene allowing blue-white screening on agar plates containing X-Gal or Bluo-Gal.
MultiShot FlexPlate DH5α T1RCompetent Cells offer:
• Transformation efficiency of >1 x 108transformants/μg plasmid DNA
•tonA (fhuA) genotype to confer resistance to T1 and T5 phage
•hsdR for efficient transformation of unmethylated DNA from PCR amplifications
•lacZΔM15 marker for blue-white screening
•endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by endonuclease I
•recA1 for reduced occurrence of non-specific recombination in cloned DNAIncrease transformation productivity with MultiShot FlexPlate formatThe FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.
Key benefits of the MultiShot FlexPlate format
•Increased throughput—compatible with automated liquid handling platforms
•Flexible—transform in increments of 8 wells to entire 96-well plate
•Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformationGenotypeF–φ80lacZΔM15 Δ(lacZYA-argF)U169recA1endA1hsdR17 (rK–, mK+)phoAsupE44 λ–thi-1gyrA96relA1tonAFind the strain and format that fits your needsMany otherE. colistrains and formats ofchemically competentandelectrocompetentcells are also available to meet specific transformation needs. For high-throughput transformation, choose from our collection ofMultiShot formatted comp cellsoremail usfor custom formatting options. We also offerbacterial growthmedia in various formats.
Antibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: No
Contains F' Episome: No
High-throughput Compatibility: High
Preparing Unmethylated DNA: No
Product Line: Multishot™
Product Type: Chemically Competent Cells
Quantity: 1 x 96 well plate (20 μl/well)
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes
Format: 96-well Plate
Species: E. coli (K12)
Unit Size: Each