Thermo Fisher, C600003, One Shot™ BL21(DE3) Chemically Competent E. coli

One Shot™ BL21(DE3) Chemically Competent E. coli One Shot BL21(DE3) Chemically CompetentE. colicells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET. This strain had been engineered to carry the λDE3 lysogen that contains T7 RNA polymerase gene under control of thelacUV5 promoter. Recombinant proteins that are nontoxic toE. coliare generally expressed at higher levels in BL21(DE3) cells compared to other strains with tighter expression control.
The BL21(DE3)E. colistrain is one of the most popular host strains to produce recombinant proteins and adapted in research or industrial applications. It is a derivative of theE. coliB strain that does not contain the lon protease and is also deficient in the outer membrane protease OmpT. The lack of two key proteases reduces degradation of heterologous proteins expressed in the strains. Additionally, several other strain features make them especially suited for protein production, namely fast growth in minimal medium, a lower acetate production when grown in high glucose media, and the ability to reach high cell density. One Shot BL21(DE3) Chemically CompetentE. colicells can achieve transformation efficiency of >1 x 108cfu/μg of control DNA.
Notes
• The basal expression levels of heterologous genes are significantly higher in BL21(DE3), thus strains such as BL21 AI are recommended for toxic protein expression.
• IPTG is required to induce expression of the T7 RNA polymerase cascade system that in turn transcribe T7 promoter regulated target gene.
One Shot BL21(DE3) Chemically CompetentE. colioffer:
• Transformation efficiency of >1 x 108cfu/μg control plasmid DNA
• Ion and OmpT protease deficiency reduces degradation of recombinant protein
• IPTG-inducible lacUV5 promoter controls expression of the T7 RNA polymerase
•hsdS mutation allows efficient transformation of unmethylated DNA
• Fast growth in minimal medium and ability to reach high cell densityEasy-to-use One Shot formatBL21(DE3) Chemically CompetentE. colicells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and prevent contamination.
Genotype:F–ompThsdSB (rB–, mB–)gal dcm(DE3)Find the strain and format that you needWe offer otherE. colistrains for protein expression. For expression of toxic proteins, considerBL21-AI™ One Shot™ Chemically CompetentE. coli.
BL21 Star (DE3) and other strains are available inMultiShot formats for high throughput applications.
Explorebacterial growthmedia formats.
We offer a variety ofsystems for the expression of recombinant proteinsinE. coli.
TheChampion pET expression systemprovides the highest level of protein production available in any expression system.
Antibiotic Resistance Bacterial: No
Blue/White Screening: No
Cloning Methylated DNA: No
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: No
Improves Protein Stability: Yes (lon,ompT)
Improves RNA Stability: No
Preparing Unmethylated DNA: Yes (dcm)
Product Line: One Shot™
Product Type: Chemically Competent Cells
Quantity: 21 x 50 μL/tube
Reduces Recombination: No
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Toxic Proteins: No
Transformation Efficiency Level: Medium Efficiency (1 x 108to 1 x 109cfu/μg)
Format: Tube
Promoter: T7
Species: E. coli (B)
Unit Size: Each